人工脂质膜重组电生理系统

• 详细介绍
• 详细参数

• 详细介绍

  德国Ionovation公司生产的Ionovation scout人工脂质膜重组电生理分析系统，是该领域最新的产品之一，该产品克服了传统膜片钳的一系列缺点，无论从产品的技术含量还是从产品的应用领域上来看，在电生理分析技术中始终处于领先的地位，代表着电生理分析技术发展的方向，是国内外细胞电生理分析实验室首选实验仪器。这种高度灵活的桌面检测系统可适应多种实验条件，目前已经被用于对细胞膜离子通道、多种动物和植物转运蛋白和它们的细胞器进行研究。

  Ionovation scout 技术特点：

  √ 不需要建立千兆欧姆的封阻

  √ 是唯一一种采用双层封阻的测量方式

  √ 可用于检测各种细胞膜上的离子通道、囊泡、配体

  √容易实现对单个离子通道进行分析检测

  √可以在膜的两侧改变条件，形成双信道进行分析              

  
  

  
  

  
  

  
  

  技术原理：

  
  

  Ionovation Scout采用了人工生物脂质膜来构建离子通道的测量方法。该系统使用两个独立的电生理测量室进行测量。该双层室由一个25μm厚、直径120微米的聚四氟乙烯隔膜隔开（a）。两个聚碳酸酯小室的体积分别为1.2 ml。聚碳酸酯小室顶部有孔，方便接入的Ag / AgCl电极和注入缓冲液（a）。当两个腔室中填充有合适的缓冲液时，溶解在正癸烷的脂质被分散到直径120微米的特氟隆隔膜的微孔（ b）中?；撼逡旱囊好婢亟档秃蜕吖?，直到特氟隆隔膜上的过量脂质被除去，特氟隆隔膜上形成脂双层自组织形成的人工生物脂质膜（C）。

  然后根据具体的目的蛋白或者细胞器等进行操作。比如类似于α-溶血素的毒素，可以直接加入聚碳酸酯小室中进行测试。而需要重组的离子通道则需要将其与脂肪酸结合为重组脂蛋白，然后将这些脂蛋白或者从生物膜上“剪下”囊泡与人工生物脂质膜通过离子梯度或融合肽方法进行融合。

  准备好样品后，开始进行电生理学测试。通过施加不同的电压，来监测细胞单离子通道的电流，其中在5-10 kHz带宽的信号会存储在系统的硬盘上，测试通常采用的模式是电压钳模式（voltage clamp mode），这种模式下应用不同的电压来得到目的信号。

  
  

  应用领域：

  1.脂质触发器

  2.细胞膜转运处理

  3.膜活性剂比如突触核蛋白

  4.药物安全分析

  5.信号转导模式分析

  
  

  
  

  
  

  应用彩神iv争霸

  Ionovation Scout测试系统可用于多种离子通道的分析:

  
  

  1. 测量对象：可实现对上万种离子通道、转运体和孔隙活动的精确测量；

  2. 软件：电生理数据采集分析软件Patchmaster，采用用户界面友好的软件实现测量全部控制；具备预定义协议式的用户定义实验工作流程；

  3. 自动操作：全自动设备操作（双层生产和验证、双层完整性的电容控制、膜双侧灌注系统）；双层分子可视化操作；

  4. 电流测量：稳定低噪声Ag/AgCl电极，采用盐桥记录电流范围从pA到几个nA

  5. 耗材：一次性双分子层室可简单、快速置换；

  
  

• 详细参数

  OVERVIEW:

   Ionovation company Ionovation scout restructuring of bilayer lipid membrane electrophysiological analysis system, is one of the latest products in this field, it overcomes the traditional patch clamp series of shortcomings, whether from the technical content of products or from the perspective of the application field of the product, in the electrophysiological analysis technology has always been in a leading position, the developing direction of the electrophysiological analysis technology, is the preferred cell electrophysiological analysis laboratory experimental apparatus at home and abroad. This highly flexible desktop detection system is adapted to a variety of experimental conditions and has been used to study cell membrane ion channels, various animal and plant transporters and their organelles.

  Ionovation scout technical characteristics：

  √ don't need to create a gigaom block

  √ the only way to measure the double seal

  √ can be used to detect ion channels, vesicles and ligands on various cell membranes

  √easy to carry out analysis and detection for single ion channel

  √The condition can be changed on both sides of the membrane to form a double channel for analysis              

  
  

  Technology principle:

  
  

        The Ionovation Scout USES artificial lipid membranes to construct ion channels. The system is measured using two separate electrophysiological measurement Chambers. The double chamber is separated (a) by a polytetrafluoroethylene septum (a) 25 mm thick and 120 micron in diameter. The volume of the two polycarbonate cells was 1.2ml. The polycarbonate chamber has holes at the top for easy access to Ag/AgCl electrodes and injection buffer (a). When the two Chambers are filled with the appropriate buffer, the lipids dissolved in the n-decane are dispersed into the pore (b) of the teflon diaphragm, which is 120 microns in diameter. The level of the buffer is continuously lowered and elevated until the excess lipids on the teflon diaphragm are removed, and an artificial biolipid membrane (C) is formed on the teflon diaphragm with a double layer of lipid self-tissue.  

        It is then manipulated according to specific target proteins or organelles. For example, a toxin similar to the hemolysin can be added directly to the polycarbonate chamber for testing. The recombinant ion channel needs to be combined with fatty acids to form recombinant lipoproteins, and these lipoproteins are then "cut" from the biofilm to fuse with the artificial lipid membrane by ion gradient or fusion peptide method.

  After the sample was prepared, the electrophysiology test was carried out. By applying different voltages to monitor the current in the cell's single ion channel, where the signal with a bandwidth of 5-10 kHz is stored on the system's hard drive, the test usually takes the form of voltage clamp mode, in which different voltages are applied to get the target signal.

  
  

  Application：

  1.Lipid triggers

  2.Cell membrane transport

  3.Membrane active agents such as synaptic nucleoprotein

  4.Drug safety analysis

  5.Signal transduction mode analysis

  
  

  Application case

  Ionovation Scout test system can be used for analysis of multiple ion channels:

  1. Measuring objects: accurate measurement of thousands of ion channels, transporters and pore activities can be achieved.

  2. Software: electrophysiological data collection and analysis software Patchmaster, which USES user-friendly software to realize all measurement control; User defined experimental workflow with pre-defined protocols;

  3. Automatic operation: full-automatic equipment operation (double-layer production and verification, double-layer integrity of capacitance control, film two-side perfusion system); Two layer visualization operation;

  4. Current measurement: stable low noise Ag/AgCl electrode, using salt bridge to record the current range from pA to several nA

  5. Consumables: one-time bimolecular chamber can be simply and quickly replaced;

  
  

• 产品特色

  因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。

• 详细介绍

  AutoCal系统提供在线的机器人校准方案，旨在快速和自动地保证机械设备的工作性能。因用于机器人各方面应用且与大多数机器人类型兼容，AutoCal系统可以检测出机器人自身构造和工具中心点（TCP）的 突然改变或偏离，并且该系统无需人为干涉就自动地更正这些误差。这意味着不用猜测哪里会出错，不用浪费宝贵时间在机器人程序重复校准上，产品品质无任何损失。

  AutoCal系统-Dynalog的先进水平校准技术，Dynalog是机器人单元标定技术的世界领导者。它的主流产品DynaCal 系统，被应用于离线的机器人单元校准，并作为最精确的和技术先进的机器人校准程序为许多机器人制造商和终端使用者所接受。AutoCal 系统将已证实的DynaCal校准技术结合到一个在线的全自动系统中，该系统专为程序控制和复原而设计的，价格低廉。